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Probiotic potentials of lactic acid bacteria isolated from Egyptian fermented food | Scientific Reports
Further, in step 5, the freeze-drying comprises the steps of, preparing liquid and filling at 20-30 ℃, putting a sample into a freeze dryer, quickly reducing the temperature of a plate layer to-40 ℃, preserving heat for 1h, when the temperature of a partition plate is-10 +/-2 ℃, preserving heat for 1h, quickly reducing the temperature of the plate layer to-40 ℃ within 1h, preserving heat for 3h, transferring to a cold trap at-50 ℃, vacuumizing to below 10Pa, firstly preserving the temperature of the plate layer at-5 ℃ for 3h, then heating the plate layer to 10 ℃ within 3h, when a product is at 0 ℃, starting to increase the temperature of the plate layer to 40 ℃, and when the product is increased to 30 ℃, preserving heat for 5 h
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Pack:Mini 30GM
EVs derived from β-cells can also affect recipient β-cells and further exert negative effects through autocrine signaling in type 2 diabetes (Wei et al., 2023)
